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PROCEDURE - CHEMICAL AND LABORATORY SAFETY
CHEMICALS AND/OR MATERIALS TO BE EXCLUDED FROM SCHOOL LABORATORIES All unlabeled bottles which contain substances of unknown composition 4-aminobipheyl Ammonium chlorate (VII) (perchlorate) Asbestos, soft forms, paper, fiber, mats, platinised, centred gauzes, gloves Benzene, used as a solvent Beryllium compounds Biphenyl-4-4' diamine (Benzedrine) Carbon monoxide Choleric (VII) acid (perchloric acid) Chloroethene (vinyl chloride monomer) Dicholorbiphenyl 1-4, 4'-diamines (chlorobenzidines) Diethyl sulphate 3,3'-dimethoxybi phenyl-4, 4'-diamines 3, 3'-dimethylbiphenyl-4, 4'-diamine (o-tolidine) Dimethyl sulphate 4, 4'-dinitrobiphenyl Ethyne cylinder (acetylene) Hydrazine Hydrofloric acid Hydrogen cynaide Mercury aklyls Naphthalen-l-amine (l-naphthylamine) Naphthalen-2-amine (2-naphthylamine) 4-nitrobiphenyl Nitrocellulose Nitrogen, triiodide Nitronaphthalenes Nitrosamines Nitrosophenols, 2- and 3- isomers Tellurium compounds Thallium and compounds Zinc chromate (VI) 9312P – Chemical and Laboratory Safety CHEMICALS EXCLUDED DUE TO SUSPECTED CARCINOGENS Aflatoxins Aminobiphenyl Arsenic compounds Asbestos Auramine (manufacture of) Benzene Benzidine Bis (chloromethyl) ether Cadmium - using, industries (possibly cadmium oxide) Chloramphenicol Chloromethyl methyl ether Chromium (chromate-producing industries) Cyclophosphamide Diethylstibestrol Hematite mining Isopropyl oils Melphalan Mustard gas 2-nathylamine Nickel (nickel refining) N,N-Bis (2 chloroethyl) -2-Naphthy-lamine Oxymetholone Phenacetin Phenytoin Soots, tars and oils Vinyl chloride 9312P – Chemical and Laboratory Safety CHEMICALS TO BE ROTATED OR RESTRICTED The following is a list of chemicals which should be either rotated on an annual basis (L) or restricted to small quantities in storage (R). Acetonitrile (methyl cyanide) Calcium phosphide Carbon tetrachloride Alcohol (other than ethanol) Aldehydes (other than methanol) R Aluminum bromide Aluminum carbide Ammonium nitrate chloromethylbenzene Chlorosulphonic acid Chromium VI oxide Benzoyl chloride 1,2 dibronopropane m-phenylenediamine p phenylenediamine Dichlorodimethylsilane Dichloroacetic acid 2,4 dichlorophenyls Bleaching powder Dodecanoyl peroxide Idoine thichloride Dimethylformamide 2-bormo-2-methylpropane 3,5-dinitrobenzoic acid 9312P – Chemical and Laboratory Safety 3-bromoprop-1-ene 2,4 dinitrophenylhydrazine Cadmium compounds Ethylene diamine Phenylammonium salts Fluorides (solid) Germanium tetrachloride Phosphorus, white Phosphorus (V) oxide Phosphorus pentabromide Hydrazine compounds Phosphorus pentachloride Phosphorus tribromide Phosphorus trichloride Hydrobromic acid Phosphorus trichloride oxide Iodine trichloride Selenium + Compounds Lithium compounds Silicon tetrachloride Methanol (formaldehyde) Methyl 2 methylpropenoate 9312P – Chemical and Laboratory Safety N-methylphenylamine Millon's reagent N-naphthylethane-1 Sulfur chlorides Naphthylthiourea Nessler's reagent Ninhydrin (solid) Titanium IV chloride Nitric acid (fuming) Uranium compounds Xylene cyanol solid 9312P – Chemical and Laboratory Safety SUGGESTED CHEMICAL STORAGE PATTERN Storage of laboratory chemicals presents an ongoing safety hazard. There are many chemicals that are incompatible with each other. The desired solution is to separate chemicals by family characteristics. Below is a list of compatible families. This arrangement will easily enable you to rearrange your inventory into a safer environment. Sulfur, Phosphorus, Arsenic Alcohols, Glycols, Etc. Phopphorus Pentoxide Halides, Sulfates, Hydrocarbons, Esters, Sulfites, Thiosulfates, Phosphates, etc. Amides, Nitrates Ethers, Kethones, etc. (not Ammonium Nitrate), Metals & Hydrides Epoxy Compounds, (Store away from any water) Hydroxides, Oxides Sulfides, Polysulfides, Arsenates, Cyanides, (Store above acids) Sulfides, Selenides, Peroxides, Azides, Phosphides, Carbides, Borates, Chromates, Acides, Anhydrides, Permanganates, etc. Chlorates, Perchlorates, Chlorites, Perchloric Acid, Peroxides, etc. Acides, except Nitric Avoid using the floor for storage of any chemicals. This list is not complete; however, should you have other unlisted chemicals, plan to take appropriate safeguards. 9312P – Chemical and Laboratory Safety LABELING CHEMICALS Put date chemical is received on label. Put on the National Fire Protection Association code. Put chemical name on label if the name has been obscured. 32 Sulfuric Acid 9 - 1981 Shelf Pattern Number Sticker: Organic Chemicals: Orange sticker on side with shelf pattern storage number on it. Inorganic Chemicals: Green sticker on side with self pattern storage number on it. 9312P – Chemical and Laboratory Safety SUGGESTED DISPOSAL PROCEDURES Each product listed in the Flinn Catalog/Reference Manual has listed under its name a disposal number. This number refers to one of the suggested disposal techniques listed below. Simply look up the product in the alphabetical section of the product listings and determine the disposal technique number. Then refer to this suggested disposal technique listing for that number and follow the suggested procedure. No representation, warranty or guarantee is made by Flinn scientific, Inc., as to the accuracy or completeness of their suggestions or information. Local conditions or circumstances unique to your particular situation (applicable federal, state or local laws) should be considered. We urgently suggest that you recognize the need to carry out these suggested procedures with proper personal safety equipment and in a proper environment. Yes, you should wear gloves, goggles and, in some cases, body protection. Yes, it would be best if these procedures were done in a fume hood. Failing that, certainly adequate ventilation should be provided or the procedure done out of doors. Stay upwind of these outdoor reactions and be certain that your activities are in an isolated area and present no hazard to your neighbors. Make a checklist of the materials you will need before you start a procedure. Flinn would suggest that you never be alone while disposing of hazardous materials and that a telephone or other form of communication be available for immediate use. For further information, write: Technical Service Department, Flinn Scientific, Inc., P.O. box #231, 910 W. Wilson Street, Batavia, Illinois 60510; or phone (312) 879-6900. #1a (ACID HALIDES, ORGANIC) #1b (HALIDES - INORGANIC) Place a layer of sodium bicarbonate in Place the material to be disposed of in the bottom of a suitable glass a large, open dish of glass, porcelain or container. Slowly add product to be plastic over a layer of sodium disposed of to this container. While bicarbonate. mixing thoroughly add this mixture to a ingredients. Using a wash bottle, spray larger container of water. While this mixture with a 6 molar solution of continuing to add large quantities of ammonium hydroxide. Smoke will water, dispose of the material down the develop and when this has diminished drain. add ice water and stir. Now place this mixture in a much larger container. It may be necessary to neutralize this strongly alkaline solution with 6 molar hydrochloric acid. Use litmus paper to determine pH of mixture and treat with acid if necessary until the mixture is neutral. After you are sure the mixture is neutralized slowly pour the material down the drain with large quantities of water. 9312P – Chemical and Laboratory Safety #4b (HALOGENS, ORGANIC) Be sure there are no sources of sparks or other sources of ignition Add the product to be disposed of to when dealing with these very vermiculite. Now add a sodium flammable materials. Assuming that carbonate-sand mixture (9 parts the quantity of material is relatively small place a generous quantity of bicarbonate). Mix these ingredients thoroughly and place in a container. Allow the vermiculite to paper bag and then in a cardboard absorb the product. Transport this carton. This material should then be material to an open and safe area burned in an open incinerator or in and burn the mixture. Use an an open area using an excelsior train excelsior train or some other safe to start the fire. method for ignition. #4c (ORGANIC ACIDS, #3 (ALKALINE EARTH METALS) This method is intended for small Place the material to be disposed of quantities of material. Cover the on a large quantity of sodium product to be disposed of with dry bicarbonate. This mixture should be soda carbonate. Remember that done in a large open vessel of glass these alkaline earth metals react or plastic. Once the mixture is vigorously with water. The sodium complete place it in a very large carbonate must be dry. Mix the bucket or drum and add a large sodium carbonate well with the quantity of water. Allow this to stand product to be disposed of while for 24 hours and then place down adding the mixture to butyl alcohol. the drain with a large quantity of Allow the mixture to stand for 24 water. Remember that you are hours being certain that it is dealing with materials that ignite so protected from disturbance. After 24 be careful to eliminate any source of hours dilute the mixture with very spark or ignition. large quantities of water and put this #5 (AMINES, AROMATIC) mixture down the drain. Make a mixture of sodium carbonate #4A (NITROPARAFFINS 7 and sand, 9 parts of sand to one part sodium carbonate. Now add the Mix the product to b disposed of with product to be disposed of to this sodium carbonate. Place this mixture and place in a paper bag mixture in a large glass or plastic and then a cardboard carton. This container. Slowly add a 6 molar material should be burned in an solution of hydrochloric acid to open incinerator or in an open area. neutralize this mixture. Use indicator Start the fire with an excelsior train. paper to know that it has been neutralized and now place the #6 (HALOGENATED AMINES, mixture down the drain with large quantities of water. 9312P – Chemical and Laboratory Safety Same as #5 #7a (AMINES, ALIPHATIC) nitrite is present. This mixture may now be disposed of down the drain Using a large open glass, porcelain with large quantities of water. or plastic dish add the material to be disposed of to a layer of sodium #9 (CARBON DISULFIDE) bisulfate. Using a wash bottle, spray this mixture with water. Neutralize You are dealing with a very flammable product here to every checking the pH with indicator paper precaution should be taken to avoid to be sure it is neutral. Now wash ignition. Using ground wire would be down the drain with large quantities a smart move in order to avoid a spark or a static charge. This material must be burned in order to #7b (PHOSPHATES, ORGANIC) dispose of it. Pour the carbon disulfide (preferably out of doors) This material will have to be over vermiculite in a suitable metal incinerated in an open and safe container. If you must transport this area. First mix the product to be material some distance to the burn disposed of with about equal parts of site then cover the carbon disulfide sand and calcium carbonate (1 soaked vermiculite with a layer of limestone). Wet this mixture down water. At the burn site pour off the with a flammable solvent (e.g., water and, using an excelsior train, alcohol) and burn it. Set the fire with ignite and burn the mixture. an excelsior train and take all precautions since you are dealing with a flammable mixture. #8 (AZO & AZIDE COMPOUNDS) Place in a very large container of water and add water until you are The material to be disposed of should be dissolved in a very large product has been neutralized. Then quantity of water until you have dump this mixture in the sewer with diluted it to a point where the large amounts of water. material being wasted represents only 5% of the mixture. Add a large #11 (INORGANIC SALTS) quantity of a 20% solution of sulfuric acid. Test with litmus so that the Fill a large glass or plastic container solution is just on the acid side. with water. Add the product to be Allow the mixture to stand for several disposed of and, in addition, add hours. Using starch-iodide indicator sodium carbonate (about 1/2 the paper check to see that slightly amount of the volume of product acidified solution will turn the paper being wasted). If a fluoride is involved also add about that same decomposition is complete since amount of calcium hydroxide. Let 9312P – Chemical and Laboratory Safety this mixture stand about 24 hours. This solution should be neutralized with 6 molar hydrochloric acid. Use indicator paper to insure that the mixture is neutral before putting the material down the drain with large quantities of water. #12a (OXIDIZING AGENTS) Add the material to be disposed of to bleachers are a 5% solution of a concentrated solution (50%) of sodium hypochlorite and they will be sodium thiosulfate (hypo). Add 3 satisfactory. This mixing and stirring molar sulfuric acid to make the should be done in a large and open solution acid. Check with indicator glass or plastic container. Allow this paper. Allow several hours to pass mixture to stand at least 12 hours. for reduction to occur. Now Check the pH with indicator paper neutralize this mixture with sodium bicarbonate or dilute hydrochloric hydrochloric acid if the mixture is acid. Dispose of the neutralized alkaline or with 6 molar ammonium material down the drain with large hydroxide solution if acid. Wash the quantities of water. neutralized solution down the drain with large quantities of water. #12b (REDUCING MATERIALS) Using a large container put the material to be disposed of with an This treatment should take place in a equal quantity of sodium carbonate large glass or plastic container. and water. A slurry will be formed. Place the product being disposed of Now add calcium hydrochlorite and in the container and make it alkaline more water. Allow this mixture to by adding a strong solution of stand for several hours. The sodium hydroxide. A slurry will form. product's pH should be determined Add to this slurry a large quantity of with indicator paper. If acid, ferrous sulfate solution. Allow about neutralize with 6 molar hydrochloric 2 hours to pass and then wash this acid solution. Wash the neutralized mixture down the drain with large material down the drain with large quantities of water. Since chlorine is quantities of water. released in this reaction it is wise to do it in a fume hood, out of doors or #13 (SULFIDES, ORGANIC & where adequate ventilation has been In some cases, you are dealing with flammable materials so take all necessary precautions. cover the Remember that you are dealing with product to be disposed of with a a very hazardous material and all 9312P – Chemical and Laboratory Safety #19 (AMIDES - INORGANIC) eliminated. Ether that is old and has been opened and exposed to light In a large glass or plastic container can contain peroxides. Just opening place a large quantity of cold water. the container can be dangerous. Add and stir in the materials to be Special disposal methods may be disposed of slowly. Allow about an required for such material. Contact hour to pass and check the pH. If us before proceeding. If you are the solution is acidic, add 3 molar satisfied that the material can be hydrochloric acid. If the solution is easily poured from its container then basic add 6 molor ammonium pour it on the ground in an isolated hydroxide. Wash the neutralized area and allow it to evaporate. solution down the drain with large quantities of water. #16 (HYDRAZINES) #20 (AMIDES - ORGANIC) Dilute the product to a 5% level or less with water in a large container. Mix the material with a quantity of Add 7 to 10 times the weight of the material being disposed of with calciuym hypochlorite. Allow to Transport to a burning site and using stand for several hours and then an excelsior train burn the material. wash down the drain with large quantities of water. #17 (HYDRIDES) Mix with dry sand and add butyl alcohol. Add a large quantity of water. Allow this mixture to stand for several hours. Pour off the liquid in to the drain with large quantities of water. Allow the sand to air dry and place it in waste container for normal disposal. #18 ALCOHOLS, KETONES, ETC.) In almost every case you are dealing with flammable material so take the necessary Absorb the material to be disposed of on vermiculite. Transport to a burning safe and construct an excelsior train and burn the material. 9312P – Chemical and Laboratory Safety #21 (INTER NON-METALLIC 24 hours and then put it down the drain with large quantities of water. Secure a quantity of dry sodium #23 (SULFIDES, INORGANIC) carbonate and calcium hydroxide. Mix the two in equal parts. Place a Make up a 30-50% solution of ferric large quantity of this mixture over the chloride solution. Add the product to material to be wasted. This should be disposed of to this solution in a be done from behind some form of large container while continuously body protection. A flash fire is stirring. Add a reasonable quantity of possible so be careful. Now spray sodium carbonate until the solution is this mixture with a large quantity of neutralized. Check the pH with water. It will be necessary that you indicator paper. After you are sure the solution is neutral dispose of the neutralize them with acid or alkali solution down the drain with large before you dispose of the material quantities of water. down the drain with large quantities #24a (ACIDS, ORGANIC) #22a (PEROXIDES, INORGANIC) Small quantities can be treated with sodium carbonate which will form a In a large glass container cover the slurry when water is added. This material with at least double its solution should be neutralized so volume in a mixture of 90% sand and that only a neutral solution is placed 10% sodium carbonate. Lumps may down the drain. Be sure to add large form so break these up with a large quantities of water. plastic spoon. Use the same spoon to then add this material slowly to another container of sodium sulfite solution. the sodium sulfite solution Hopefully the amount involved is should be in a volume of 4-5 liters. small and the technique is only Neutralize this final solution with intended for small amounts. Take dilute sulfuric acid. Check to see the material to a safe and open site that it's neutralized with indicator and slowly add it to a very large paper. Drain the solution into the container of water. Since a sewer with large quantities of water. hydrocarbon gas will be generated Air dry the sand and dispose of it in a no source of ignition should be present. Allow the material to stand for at least 24 hours. A precipitate #22b (PEROXIDES, ORGANIC) will be formed. Pour off the liquid and dispose of the remaining This method is intended for only precipitate in a normal disposal small quantities of materials. Add 10 times the amount of material to be disposed of with a 20% sodium #26 (WASTE FOR LANDFILL) hydroxide solution. allow to stand for 9312P – Chemical and Laboratory Safety disposed of at a local landfill and #27c (PHOSPHORUS) present little or no hazard. If the quantity is small simply cover #27a (SCRAP METALS) the material with water and take to a These materials can be safely open area. The water will evaporate disposed of in a landfill but this and the dry phosphorus will ignite separate procedure is listed since spontaneously in air and burn up. the metals may have scrap value on your local market and this should be #27d, #27f, #27h investigated before disposing of potentially valuable materials. Use procedure described under Only the most severely contaminated mercury cannot be recovered. Place the materials involved cannot be all contaminated mercury in a very burned and are not soluble in water. For small amounts the materials can closed bottle and contact Flinn simply be allowed to evaporate out Scientific or your other chemical of doors. For large quantities we suppliers for possible recovery. For suggest you contact your supplier for very small quantities of material add possible return of the material. zinc metal powder which will form an amalgam that can be disposed of in your normal waste. The nature of mercury and mercury compounds is These materials can be burned in such at none should be disposed of your incinerator as would any normal in the environment. paper, wood or other wastes. INSTANT WARNING SYSTEM FOR DANGEROUS MATERIALS The National fire Protection Association (NFPA) has developed a numerical system for the identification of the fire hazards by materials. The numbers given in the three columns have been taken from NFPA 704M-6th edition. 9312P – Chemical and Laboratory Safety Abbreviated definitions are as follows: 4 Can cause death or major injury despite medical treatment. 3 Can cause serious injury despite medical treatment. 2 Can cause injury. Requires prompt treatment. 1 Can cause irritation if not treated. 0 No hazard. FLAMMABILITY (RED) 4 Very flammable gasses or very volatile flammable liquids. 3 Can be ignited at all normal temperatures. 2 Ignites if moderately heated. 1 Ignites after considerable preheating. 0 Will not burn. REACTIVITY (STABILITY) (YELLOW) 4 Readily detonates or explodes. 3 Can detonate or explode but requires strong initiating force or heating under confinement. 2 Normally unstable but will not detonate. 1 Normally stable. Unstable at high temperature and pressure. Reacts with water. 0 Normally stable. Not reactive with water. Please refer to the Policies & Procedures Manual for additional information. Implemented: North Thurston School District See Policies and Procedures manual for additional information.

Source: http://www.nthurston.k12.wa.us/cms/lib/WA01001371/Centricity/Domain/9/proc9312P.pdf

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ISSN: 1536·8378 (print), 1536-8386 (electronic) Electromagn Bioi Med, Early Online: 1-12 AND MEDICINE © 2013 lnforma Healthcare USA, Inc. DOl: 10.3109/15368378.2013.817334· ORIGINAL ARTICLE Extra-low-frequency magnetic fields alter cancer cells through metabolic restriction Ying Li1 2 and PauI Heroux 2 'lnVitroPJus Laboratol'f, Department of Surgel'f, Royal Victoria Hospital,Montreal, QC,Canada and 2Department of Epidemiology, Biostatistics and

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